HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Full Text (PDF) Supplemental material Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Johnson, G. A. Articles by Hedlund, L. W. Search for Related Content PubMed PubMed Citation Articles by Johnson, G. A. Articles by Hedlund, L. W.

Morphologic Phenotyping with MR Microscopy: The Visible Mouse¹

¹ From the Center for In Vivo Microscopy, Duke University Medical Center, Rm 141, D Bryan Neuroscience Bldg, Research Dr, Durham, NC 27710. Received March 5, 2001; revision requested April 14; final revision received September 5; accepted September 6. Supported by the National Institutes of Health National Center for Research Resources (P41 RR05959). Address correspondence to G.A.J. (e-mail: gaj@orion.mc.duke.edu).

View larger version (91K): [in a new window]   Figure 1. Coronal 2-mm-thick sections were acquired in (A) a formalin-fixed specimen and (B) a specimen stained with a 1:20 mixture of gadopentetate dimeglumine and formalin. At TR of 100 msec, the gain in signal-to-noise ratio is fivefold greater than that for all tissues except fat.

View larger version (62K): [in a new window]   Figure 2. Graph shows the T1 calculated for seven representative tissues in stained (1:20 Gd) and unstained (control) specimens. The T1 was reduced by more than sixfold in all tissues except fat. Gd = gadopentetate dimeglumine.

View larger version (114K): [in a new window]   Figure 3. Three-dimensional MR microscopic images with an isotropic array (256 x 256 x 1,024) were acquired in a whole fixed C57BL/6J mouse. The spatial resolution in every plane is 110 x 110 x 110 µm, which allows one to view any arbitrary plane with equal spatial resolution. Coronal (top) and transverse (bottom) images depict 110-µm-thick sections of the head (left), thorax (center), and abdomen (right).

View larger version (120K): [in a new window]   Figure 4. Coronal 3D (512 x 512 x 512) images of control (left) and uricase knockout (right) mice permit morphologic phenotyping. Isotropic resolution of 50 µm allows identification of liver (a), stomach (b), right kidney cortex (c), outer medulla (d), inner medulla-papilla (e), and left kidney cortex (f). In the uricase knockout mouse (right), kidneys (g) are hydronephrotic with cortical and medullary cysts (h). The use of 3D isotropic sampling makes it easy to extract corresponding sections from the two image volumes for comparison.

View larger version (86K): [in a new window]   Figure 5. Images of the right kidney that has been removed from a perfusion-fixed mouse and imaged with a 256 x 512 x 512 array, resulting in 25-µm-thick voxels. In addition to the thinnest 25-µm-thick section of the kidney (A), rendering techniques can produce a 250-µm-thick section (B), and a 2,500-µm-thick slab (C), in which nonvascular tissue is transparent. Because the specimen was not physically sectioned, 3D anatomic relationships such as the branching vasculature are undisturbed.