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Effect of Human Stem Cells Labeled with Ferumoxides–Poly-L-lysine on Hematologic and Biochemical Measurements in Rats¹

¹ From the Experimental Neuroimaging Section, Laboratory of Diagnostic Radiology Research, Clinical Center, National Institutes of Health, Bethesda, Md. Received February 27, 2004; revision requested May 5; revision received June 2; accepted July 1. Address correspondence to A.S.A., Radiology Research, Henry Ford Health System, 1 Ford Pl, 2F, Box 82, Detroit, MI 48188 (e-mail: saali@rad.hfh.edu).

View larger version (70K): [in a new window]   Figure 1. Mean hematologic measurements (± standard deviations) in rats. Total white blood cell (WBC) counts (A), hemoglobin concentrations (B), platelet counts (C), MCVs (D), lymphocyte differentials (E), and monocyte differentials (F) measured during a 42-day postinjection period in three to five blood samples from rats injected with labeled MSCs (black bars) and ferumoxides-PLL complex (white bars). Day 14 measurements were omitted owing to insufficient data (fewer than three samples obtained). * = measurements significantly different from control values (P < .05), # = significant differences between labeled MSC injection and ferumoxides-PLL complex injection data (P < .05). K/µL = 1000 cells per microliter, 10k/µL = 10 000 cells per microliter.

View larger version (50K): [in a new window]   Figure 2. Mean blood chemistry measurements (± standard deviations). Alkaline phosphatase (A), alanine aminotransferase (ALT) (B), AST (C), and direct bilirubin (D) concentrations measured during a 42-day postinjection period in three to five blood samples from rats injected with labeled MSCs (black bars) and ferumoxides-PLL complex (white bars). Data, including AST control values, were omitted if an insufficient number of samples (fewer than three) were available. At ANOVA, no significant differences in values were observed during the 42-day period in the rats injected with labeled MSCs, whereas differences in alkaline phosphatase, alanine aminotransferase, and AST values were observed in the rats injected with ferumoxides-PLL complex. * = measurements significantly different from control values (P < .05), # = significant differences between labeled MSC injection and ferumoxides-PLL complex injection data (P < .05).

View larger version (105K): [in a new window]   Figure 3. Photomicrographs of blood samples obtained from a group 4 rat 1 minute (A), 5 minutes (B), 15 minutes (C), 30 minutes (D), 1 hour (E), and 2 hours (F) after intravenous injection of ferumoxides-PLL-labeled MSCs (with red blood cells lysed and removed). Compared with blood cells, labeled MSCs are large and blue, indicating the presence of iron. At 15 minutes after injection, no MSCs appear to remain in the circulation. (Prussian blue stain; original magnification, x10.)